PREDICTION OF EXTERNAL PANCREATIC FISTULA DEVELOPMENT IN PATIENTS WITH ACUTE INFECTED NECROTISING PANCREATITIS.

OBJECTIVE: The aim: To search for risk factors for the development of external pancreatic fistula (EPF) in patients with acute infected necrotizing pancreatitis. PATIENTS AND METHODS: Materials and methods: A prospective single-center observational study was conducted with the participation of 160 adult patients with infected acute necrotizing pancreatitis (ANP) who required intervention at different stages of the disease. Depending on the amylase activity of the drainage secretions, the groups with and without diagnosed EPF were compared according to 15 demographic, clinical, laboratory and instrumental parameters of the disease. To identify predictors of the risk of developing EPF in ANP, a regression logistic analysis was performed and logistic regression models were built for each factor attribute. RESULTS: Results: We did not find statistically significant differences in the indicators characterising patients on the first day of illness, as well as in the comparison of indicators characterising local complications of AP, the timing of AP infection, the incidence of sepsis and the level of PON in sepsis. When comparing the groups by the frequency of the type of microbial agent of infected APB, no statistically significant differences were found. Comparative pairwise analysis between the groups by morphological characteristics revealed statistical differences in the frequency of focal superficial and transmural PN (p < 0.001). Next, we performed a regression logistic analysis and built logistic regression models for each factor attribute, namely age, gender, BMI, BISAP score and Charlson comorbidity index, morphological characteristics of necrotizing pancreatitis. After univariate regression analysis, a statistically significant association between the depth of PN and the risk of developing PFN was found (OR 2.7 (1.4-5.2), p=0.001). CONCLUSION: Conclusions: We found that the risk of developing an external pancreatic fistula was associated with the depth of pancreatic necrosis.

ABDOMINAL PERFUSION PRESSURE IN PREDICTION OF THE TERMS OF ACUTE NECROTIZING PANCREATITIS INFECTION.

OBJECTIVE: The aim: To determine the role of the abdominal perfusion pressure level at the beginning of the disease in predicting the timing of infection of acute nec¬rotizing pancreatitis. PATIENTS AND METHODS: Materials and methods: A retrospective cross-sectional single-center study of 39 patients with acute severe pancreatitis (ASP) and pancreatic infection (PI) with measured and calculated minimal abdominal perfusion pressure (APP) in the first 48 hours of the disease. The existence of a correlation between the onset of PI and the level of intra-abdominal (IAP) and abdominal perfusion pressure was calculated and a single-factor mathematical model of linear regression was built. RESULTS: Results: A negative strong correlation was found between IAP and the timing of PI onset, ρ=-0.818 (p<0.001), and a positive strong correlation was found between APP and the timing of PI onset, ρ=0.933 (p<0.001). The dependence of the outcome variable (PI term) on the APP value was revealed, adjusted coefficient of determination R²adj = 0.887 was corrected, F value 288.5, p<0.001, root mean square errors RSE = 1.31 (with 37 degrees of freedom). The value of the coefficient X1 was 0.47±0.0, p<0.001. CONCLUSION: Conclusions: An increase in IAP in the first 48 hours of the desease in patients with ASP was, on average, accompanied by a reduction in the start of PI. A decrease in APP in the first 48 hours of the desease in patients with ASP was, on average, accompanied by a reduction in the start of PI. In the study sample, it was possible to predict the time of PI initiation by measuring the APP level in the first 48 hours of the disease with a margin of error for 1.3 days. It was found that with a decrease in the APP level, the time of the PI occurrence was reduced (p<0.001), on average, by 0.47±0.02 days for each mm Hg of APP.

CHANGES ANALYSIS OF THE HEPATOCYTE APOPTOSIS MARKERS LEVELS IN MALIGNANT OBSTRUCTIVE JAUNDICE COMPLICATED BY CHOLANGITIS.

OBJECTIVE: The aim: To evaluate changes in the levels of hepatocyte apoptosis markers in malignant obstructive jaundice (MOJ) depending on the performance of preoperative biliary decompression (PBD) and the severity degree of primary ascending cholangitis (PAC). PATIENTS AND METHODS: Materials and methods: 136 patients with MOJ complicated by cholangitis were included in the study: group A (n=84) - patients who underwent PBD; group B (n=52) - patients without PBD. The level of CASP3 and Bcl-2 (Human Bcl-2(B-cell Leukemia/Lymphoma 2) in blood serum and bile was assessed according to the principle of Sandwich-ELISA. Material collection for research was performed at the PBD stage and intraoperatively. RESULTS: Results: Comparative analysis of CASP3 levels in patients of the study groups revealed that the level of this indicator in the blood and bile of group A patients was statistically significantly higher compared to group B, p=0,004 and p<0,001, respectively. There was no statistically significant difference between the study groups in the intraoperative levels of blood serum Bcl-2 (p=0,786) and bile Bcl-2 (p=0,439). The presence of a correlation between apoptosis markers in group A patients with I and II degree of PAC at the time of PBD and the main surgical intervention was determined: blood serum CASP3 - r=0,733, p<0,001 and r=0,753, p<0,001; bile CASP3 - r=0,716, p<0,001 and r=0,792, p<0,001; blood serum Bcl-2 - r=0,609, p<0,001 and r=0,495, p=0,002; bile Bcl-2- r=0,744, p<0,001 and r=0,497, p=0,002, respectively. Binary logistic regression analysis showed that the development of grade I and II PAC did not relate with the levels of apoptosis markers (p>0.05). Linear regression analysis revealed a correlation between the levels of Bcl-2 in bile during PBD and intraoperatively in group A patients with moderate grade OJ (R2=0,547, p<0,001) and between the levels of CASP3 in blood serum (R2=0,614, p<0,001), CASP3 in bile (R2=0,603, p<0,001), Bcl-2 in blood serum (R2=0,484, p<0,001) and Bcl-2 in bile (R2=0,485, p<0,001) in PBD and intraoperatively in patients with severe grade OJ. A statistically significant difference in the levels of Bcl-2 in blood serum (p<0,001) and Bcl-2 in bile (p=0,016) was found when comparing apoptosis markers in patients with moderate grade OJ of the study groups. Binary logistic analysis showed that the performance of PBD had a significant (reducing) effect on CASP3 levels in blood serum and bile taken intraoperatively in study groups patients with moderate grade OJ (R2= 0,292, p<0,001; R2= 0,184, p<0,001). CONCLUSION: Conclusions: Prolonged OJ leads to the pathological apoptosis process. The performance of PBD statistically significantly reduces the level of CASP3 in blood serum and bile, which is confirmed by further determination intraoper¬atively in patients with OJ complicated by PAC, p<0,001. Staged surgical intervention with the performance of PBD according to clear indications is a necessary treatment strategy in patients with MOJ complicated by cholangitis.

C-REACTIVE PROTEIN AS A MARKER OF CLINICAL AND LABORATORY REMISSION IN PATIENTS WITH ACUTE NECROTIZING PANCREATITIS.

OBJECTIVE: The aim: To estimate the role of C-reactive protein levels as a marker of clinical and laboratory remission in patients with acute necrotizing pancreatitis. PATIENTS AND METHODS: Materials and methods: A single-center retrospective non-randomized study of 68 patients (37 (54%) men, 31 (46%) women) was carried out in the period from September 2019 to October 2020. The patients were divided into two groups: group 1 included 9 (13.2%) patients rehospitalized within a month of observation after being discharged from hospital with improvement, group 2 consisted of 59 (86.8%) patients not readmitted within the observation period. A standard package of Microsoft Excel, program MedStat v.5.2, W-test Wilcoxon and the Fisher transform was used to record, calculate and compare data. RESULTS: Results: Patients in groups 1 and 2 did not statistically differ in age (p = 0.727), gender (p = 0.202), body mass index (BMI) (p = 0.447), length of hospital stay (p = 0.913), volume of pancreatic lesion according to СTSI (p = 0.313) and severity of disease (p = 0.205). Incidence of pancreatic necrosis infection was 88.8% (p = 0.007) in group 1 versus 35.6% in group 2. In the group of rehospitalized patients, the level of C-reactive protein upon discharge was significantly higher (Me ± m 80.8± 9.734 (66) -88), CI 95%) compared with the patients from the group without readmission (Me ± m 21.36± 2.285 (16.1-31.1) CI 95%) p <0.001. As the "critical" CRP level for group 1, indicating readmission for infectious complications of AP, was chosen the value of the left limit of 95% CI (confidence interval), which made up ≥64.5 mg / ml. CONCLUSION: Conclusions: According to the results of our study, the risk of readmission in patients with CRP level ≥64.5 mg / L prior to being discharged exceeded 41 times the risk of readmission in those with lower CRP values (RR 41.5 ± 1.008 (95% CI 5.75-299, p = 0.04)). For each patient with CRP levels ≥64.5 mg / L, the risk of readmission within the first month after being discharged was 71% (95% CI 40.7-88.6, p = 0.03).

CASE STUDY: MAJOR DUODENAL PAPILLA CANCER COMPLICATED BY ACUTE PARACANCROTIC NECROTIZING PANCREATITIS.

The given paper describes a case of treating adenocarcinoma of the papilla of Vater diagnosed as the cause after an episode of acute pancreatitis. The etiology of aсute pancreatitis was considered idiopathic until the onset of complaints caused by tumor growth. The volume of radical surgery has changed intraoperatively due to the detection of infected limited necrotic clusters, didn't diagnosed both laboratory and instrumentally. Pathomorphological conclusion: considering clinical data, low-grade (G3) adenocarcinoma of the major duodenal papilla, which developed from pre-existing tubular villous adenoma of the duodenum or intraampullary papillary neoplasia (IAPN). Predicting the early detection of MNPs after AP episode requires further research by improving imaging techniques, introducing new non-invasive techniques and investigating circulating biomarkers. The scope of surgery for patients with clinically significant complications of tumor growth and identified competing diagnosis at the target site should be individually decided and weighed. Infected pancreatitis in the late stages requires careful diagnosis.